The Influence of Dahlia Tubers Extract (Dahlia Variabilis) on Diabetic in Rats
DOI:
https://doi.org/10.22399/ijcesen.4040Keywords:
Body Weight, Diabetes Mellitus, Dahlia Bulbs, Insulin, Blood GlucoseAbstract
Diabetes mellitus is a chronic metabolic problem disease defined by hyperglycemia that is recognized as a silent killer and the mother of the disease, causing complications and endangering health. Inulin is a natural fiber found in dahlia tubers (dahlia variabilis) that functions as a dietary fiber and is suitable for diabetics to consume. The purpose of this study was to see how inulin from dahlia tubers affects blood glucose levels in diabetic rats. The rats were randomized into five groups at random: normal control, STZ-induced diabetic, STZ-induced diabetic with inulin (0.5 mg/g BW), STZ-induced diabetic with inulin (1.0 mg/g BW), and STZ-induced diabetes (1.5 mg/g BW). Before and after treatment, rats' blood glucose levels were assessed. This study discovered that inulin doses of 0.5mg/g BW, 1.0mg/g BW, and 1.5mg/g BW lowered rats' fasting blood glucose levels. When compared to the other treatment groups, the rats administered inulin at a dose of 1.5mg/g BW demonstrated the most significant drop in fasting blood glucose levels (p<0.05). The reduction in fasting blood glucose levels of rats in the treatment group was accompanied by a substantial reduction in body weight compared to the normal control group (p<0.05) at the end of the treatment. From the study, it was found that administration of inulin from dahlia tuber extract at a dose of 1.5 mg/g BW could significantly reduce blood glucose levels and body weight in diabetic rats.
References
[1] Wediasari F, Nugroho GA, Fadhilah Z, Elya B, Setiawan H, Mozef T. Hypoglycemic Effect of a Combined Andrographis paniculata and Caesalpinia sappan Extract in Streptozocin-Induced Diabetic Rats. Adv Pharmacol Pharm Sci. 2020;2020.
[2] Zargar AH, Balagopalan JP, Bhattacharyya A, Almeida A, Taraphder A, Bansal S, et al. Diabetes-Kidney-Heart Continuum and Its Implication on Therapeutic Management. Cureus. 2025;17(7):E88561.
[3] Abate TW, Genanew A, Gedamu H, Tegenaw A, Ayalew E, Berhie AY, et al. Unmasking the silent epidemic : a comprehensive systematic review and meta-analysis of undiagnosed diabetes in Ethiopian adults. Front Endocrinol (Lausanne). 2024;15:1372046.
[4] International Diabetes Federation. IDF Diabetes Atlas [Internet]. 11th ed. J D, Magliano, J E, Boyko, Genitsaridi I, Piemonte L, et al., editors. Brussels, Belgium: International Diabetes Federation; 2025. 1–131 p. Available from: https://diabetesatlas.org/resources/idf-diabetes-atlas-2025.
[5] International Diabetes Federation. IDF. 2025. Indonesia diabetes trends and prevalence. Available from: https://diabetesatlas.org/data-by-location/country/indonesia/.
[6] Costa PA, Souza DC de, Ossani PC, Mendes MHA, Silva ML de S, Carvalho E, et al. Nutritional and functional compounds in dahlia flowers and roots. Brazilian J Food Technol. 2022;25.
[7] Aldubayan MA, Mao X, Laursen MF, Pigsborg K, Christensen LH, Roager HM, et al. Supplementation with inulin-type fructans affects gut microbiota and attenuates some of the cardiometabolic benefits of a plant-based diet in individuals with overweight or obesity. Front Nutr. 2023;10:1108088.
[8] Li J, Liu F, Luo Y, Wijeyesekera A, Wang S, Chen X, et al. Differential effects of inulin and fructooligosaccharides on gut microbiota composition and glycemic metabolism in overweight / obese and healthy individuals : a randomized , double ‑ blind clinical trial. BMC Med. 2025;23(372):1–17.
[9] He J, Li X, Yan M, Chen X, Sun C, Tan J, et al. Inulin Reduces Kidney Damage in Type 2 Diabetic Mice by Decreasing Inflammation and Serum Metabolomics. J Diabetes Res. 2024;2024:1–14.
[10] He J, Li X, Yan M, Chen X, Sun C, Tan J, et al. Inulin Reduces Kidney Damage in Type 2 Diabetic Mice by Decreasing Inflammation and Serum Metabolomics. J Diabetes Res. 2024;2024:1–14.
[11] He J, Li X, Yan M, Chen X, Sun C, Tan J, et al. Inulin Reduces Kidney Damage in Type 2 Diabetic Mice by Decreasing Inflammation and Serum Metabolomics. J Diabetes Res. 2024;2024:1–14.
[12] Giuberti G, Gallo A. Heliyon In vitro evaluation of fermentation characteristics of type 3 resistant starch. Heliyon [Internet]. 2020; 6 (December 2019): e03145. Available from: https://doi.org/10.1016/j.heliyon.2019.e03145.
[13] Yusuf BO, Okeme U, Akinfenwa AO, Adeola I, Bisiriyu ZA, Lukman HY, et al. Control of diabetic nephropathy in male Wistar rats using ethanolic extract of Monodora myristica seeds and its biosynthesized selenium nanoparticles. Explor Drug Sci. 2025;2025(3):100889.
[14] Karolinne A, Victória A, Acha BT, Suellenn A, Oliveira S, Macedo JL, et al. Experimental Models of Type 2 Diabetes Mellitus Induced by Combining Hyperlipidemic Diet (HFD) and Streptozotocin Administration in Rats : An Integrative Review. Biomedicines. 2025;13:1158.
[15] Cruz PL, Moraes-silva IC, Ribeiro AA, Machi JF, Melo MDT de, Santos F dos, et al. Nicotinamide attenuates streptozotocin- induced diabetes complications and increases survival rate in rats : role of autonomic nervous system. BMC Endocr Disordes. 2021;21(133):1–10.
[16] Ghasemi A, Jeddi S. Review article : Sterptozotocin as a Tool for Induction of Rat Models of Diabetes: a Practical Guide. EXCLI J. 2023;22:274–294.
[17] Ghavidel F, Amiri H, Tabrizi MH, Alidadi S, Hosseini H, Sahebkar A. The Combinational Effect of Inulin and Resveratrol on the Oxidative Stress and In fl ammation Level in a Rat Model of Diabetic Nephropathy. Curr Dev Nutr [Internet]. 2024;8(1):102059. Available from: https://doi.org/10.1016/j.cdnut.2023.102059.
[18] Wahyuwardani S, Praharani L, Noor SM, Bakrie B, Widiyanti PM. Assessing the knowledge of ethical clearance and animal welfare among researchers in Indonesia : A cross-sectional study. Vet World. 2025;18(8):2499–2510.
[19] Rehman H ur, Ullah K, Rasool A, Manzoor R, Yuan Y, Tareen AM, et al. Comparative impact of streptozotocin on altering normal glucose homeostasis in diabetic rats compared to normoglycemic rats. Sci Rep [Internet]. 2023;13(7921):1–6. Available from: https://doi.org/10.1038/s41598-023-29445-8.
[20] Titisari N, Ahmad H, Samsulrizal N, Fauzi A, Razak ISA. The mechanism underlying streptozotocin injection for the development of a nontransgenic Alzheimer ’ s disease animal model. Open Vet J. 2025;15(2):594–600.
[21] Ismawati I, Saryono S, Mukhyarjon M, Romus I, Putri VD, Yanti S, et al. Effect of Inulin from Dahlia Tubers (Dahlia variabilis) Extract on Insulitis Severity and Insulin Expression in Diabetic Rats. BioMedicine. 2024 Aug 30;14(3):31–39.
[22] Aliasgharzadeh A, Khalili M, Mirtaheri E, Gargari BP, Tavakoli F, Farhangi MA, et al. A combination of prebiotic inulin and oligofructose improve some of cardiovascular disease risk factors in women with type 2 diabetes: A randomized controlled clinical trial. Adv Pharm Bull. 2015;5(4):507–514.
[23] Dehghan P, Gargari BP, Asgharijafarabadi M. Effects of High Performance Inulin Supplementation on Glycemic Status and Lipid Profile in Women with Type 2 Diabetes: A
Randomized, Placebo-Controlled Clinical Trial. Heal Promot Perspect. 2013;3(1):55–63.
[24] Giuntini EB, Sardá FAH, Menezes EW de. The Effects of Soluble Dietary Fibers on Glycemic Response : An Overview and Futures Perspectives. Foods. 2022;11(3934):1–26.
[25] Kumar A, Prajapati P, Raj V, Kim SC, Mishra V, Raorane CJ, et al. Salbutamol ameliorates skeletal muscle wasting and inflammatory markers in streptozotocin (STZ)-induced diabetic rats. Int Immunopharmacol. 2023;124(Part A):110883.
[26] Rahmati M, Taherabadi SJ. The effects of exercise training on Kinesin and GAP ‑ 43 expression in skeletal muscle fibers of STZ ‑ induced diabetic rats. Sci Rep [Internet]. 2021;11(9535):1–9. Available from: https://doi.org/10.1038/s41598-021-89106-6.
[27] Ilda H, Watada H. Pancreatic β -cell Dysfunction and Diabetes. Juntendo Med J. 2025;71(3):158–165.
[28] Dludla P V, Mabhida SE, Ziqubu K, Nkambule BB, Mbeje SEM, Hanser S, et al. Pancreatic β-cell dysfunction in type 2 diabetes: Implications of inflammation and oxidative stress. World J Diabetes. 2023;14(3):130–146.
[29] Li L, Li P, Xu L. Assessing the effects of inulin- type fructan intake on body weight, blood glucose, and lipid profile: A systematic review and meta- analysis of randomized controlled trials. Food Sci Nutr. 2021;2021(9):4598–4616.
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